UArizona DataLab Special Workshop: RNA-Seq

Instructors: Wesley Brashear and Joshua Winchell, High-Performance Research Computing, Texas A&M University.

Key Topics Covered:

• Experimental Design: Learn how to strategize your RNA-Seq experiments for optimal results, considering factors like sample preparation, library preparation, and sequencing depth.
• Sequencing Strategies: Explore the latest techniques and technologies in RNA-Seq, including single-cell, bulk, and long-read sequencing approaches.
• Data Processing: In-depth knowledge of the command line, and essential tools for quality control, read mapping, and data preprocessing.
• Differential Expression Analysis: Perform differential gene expression analysis using state-of-the-art R packages and statistical models.
• Visualization and Interpretation: Develop data storytelling skills for more informative visualizations.

Prerequisites:

• Basic Linux skills and, preferably
• Familiarity with Next Generation Sequencing data formats (e.g. FASTQ files, SAM/BAM files)

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